People > Marcelo Garcia
Environmental Engineering D.Sc. Student
Research: Unraveling the syntrophic acetate oxidizing bacteria
in anaerobic digesters treating swine waste.
The overall goal of the
proposed study is to improve stability of farm-based anaerobic digestion
treating swine waste. The supporting objectives are: 1.
to perform hypothesis-driven research to understand the role of syntrophic
bacteria in anaerobic digestion stability; 2. to develop
innovative techniques, which will be used to study the syntrophic bacteria
in anaerobic digestion systems ; and 3. to validate results
with full-scale systems. To improve the stability of anaerobic
digesters a mechanistic understanding of the microbial population dynamics
is required and such information can only be found when utilizing culture-independent
molecular biology techniques to classify and quantify syntrophic
bacteria. Lab-scale anaerobic sequencing batch reactor (ASBR),
serum bottle, and micro-bioreactor systems will be operated to ascertain
if syntrophic bacteria are below a critical threshold or too
sensitive to perturbations for a stable operation of anaerobic
digesters treating swine waste at high-ammonia-N levels.
